Isolation and characterization of salt-associated protein in Citrus
Identifieur interne : 003788 ( Main/Exploration ); précédent : 003787; suivant : 003789Isolation and characterization of salt-associated protein in Citrus
Auteurs : Gozal Ben-Hayyim [Israël] ; Zehava Faltin [Israël] ; Shimon Gepstein [Israël] ; Luc Camoin [France] ; A. Donny Strosberg [France] ; Yuval Eshdat [Israël]Source :
- Plant Science [ 0168-9452 ] ; 1993.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
Abstract
A significant increase in the amount of a protein, whose migration in two-dimensional gel electrophoresis corresponds to an apparent olecular weight of 23–25 kDa and pI=6.1, was observed in adapted salt-tolerant cultured cells derived from Shamuti orange (Citrus sinesis L. Osbeck) ovular callus cells. An increase was also determined when these cells were grown in the presence of abscisic acid (ABA) or polyethylene glycol (PEG) instead of NaCl, similarly to what was previously observed for osmotin, the tobacco salt-associated protein. However, no similarity has been so far observed between these proteins in their biochemical and immunochemical properties. Pulse labeling of the salt-tolerant cells suggests a low turnover of the accumulated protein. Its salt-induced accumulation in the cells was also relatively slow and gradually increased during a period of several days following exposure of the cells to NaCl. Cell fractionation experiments suggest that the protein is allocated in the soluble fraction and is not an integral membrane protein. Examination of the presence of the protein in citrus plants, such as Etrog citron (C. medicaL.) and Cleopatra mandarin (C. reticulata) cultivars, revealed an increase in its concentration in all organs examined, following irrigation of the plants with saline water. Thus, this protein is associated with stress conditions employed on growing plants as well and is not a unique phenomenon restricted to cultured cells only.
Url:
DOI: 10.1016/0168-9452(93)90084-D
Affiliations:
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Donny Strosberg</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire d'Immuno-Pharmacologie Moléculaire, CNRS, Université Paris VII and Institut Cochin de Génetique Moléculaire, 22 rue Méchain, 75014 Paris</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Eshdat, Yuval" sort="Eshdat, Yuval" uniqKey="Eshdat Y" first="Yuval" last="Eshdat">Yuval Eshdat</name><affiliation wicri:level="1"><country xml:lang="fr">Israël</country><wicri:regionArea>Department of Friut Tree Breeding and Genetics, Agricultural Research Organization, The Volcani Center, 50250 Bet Dagan</wicri:regionArea><wicri:noRegion>50250 Bet Dagan</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Plant Science</title><title level="j" type="abbrev">PSL</title><idno type="ISSN">0168-9452</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1993">1993</date><biblScope unit="volume">88</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="129">129</biblScope><biblScope unit="page" to="140">140</biblScope></imprint><idno type="ISSN">0168-9452</idno></series><idno type="istex">E62C7DE9F4E5580E2F6E39FFB07AD689D1394870</idno><idno type="DOI">10.1016/0168-9452(93)90084-D</idno><idno type="PII">0168-9452(93)90084-D</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0168-9452</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell culture</term><term>Citrus sinensis</term><term>Gel electrophoresis</term><term>Gene expression</term><term>Osmolarity</term><term>Osmotic shock</term><term>Proteins</term><term>Salinity</term><term>Salt resistance</term><term>Stress</term><term>Tolerance</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Choc osmotique</term><term>Citrus sinensis</term><term>Culture cellulaire</term><term>Electrophorèse gel</term><term>Expression génique</term><term>Osmolarité</term><term>Protéine</term><term>Résistance sel</term><term>Salinité</term><term>Stress</term><term>Tolérance</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A significant increase in the amount of a protein, whose migration in two-dimensional gel electrophoresis corresponds to an apparent olecular weight of 23–25 kDa and pI=6.1, was observed in adapted salt-tolerant cultured cells derived from Shamuti orange (Citrus sinesis L. Osbeck) ovular callus cells. An increase was also determined when these cells were grown in the presence of abscisic acid (ABA) or polyethylene glycol (PEG) instead of NaCl, similarly to what was previously observed for osmotin, the tobacco salt-associated protein. However, no similarity has been so far observed between these proteins in their biochemical and immunochemical properties. Pulse labeling of the salt-tolerant cells suggests a low turnover of the accumulated protein. Its salt-induced accumulation in the cells was also relatively slow and gradually increased during a period of several days following exposure of the cells to NaCl. Cell fractionation experiments suggest that the protein is allocated in the soluble fraction and is not an integral membrane protein. Examination of the presence of the protein in citrus plants, such as Etrog citron (C. medicaL.) and Cleopatra mandarin (C. reticulata) cultivars, revealed an increase in its concentration in all organs examined, following irrigation of the plants with saline water. Thus, this protein is associated with stress conditions employed on growing plants as well and is not a unique phenomenon restricted to cultured cells only.</div></front></TEI><affiliations><list><country><li>France</li><li>Israël</li></country><region><li>Île-de-France</li></region><settlement><li>Paris</li></settlement></list><tree><country name="Israël"><noRegion><name sortKey="Ben Hayyim, Gozal" sort="Ben Hayyim, Gozal" uniqKey="Ben Hayyim G" first="Gozal" last="Ben-Hayyim">Gozal Ben-Hayyim</name></noRegion><name sortKey="Eshdat, Yuval" sort="Eshdat, Yuval" uniqKey="Eshdat Y" first="Yuval" last="Eshdat">Yuval Eshdat</name><name sortKey="Faltin, Zehava" sort="Faltin, Zehava" uniqKey="Faltin Z" first="Zehava" last="Faltin">Zehava Faltin</name><name sortKey="Gepstein, Shimon" sort="Gepstein, Shimon" uniqKey="Gepstein S" first="Shimon" last="Gepstein">Shimon Gepstein</name></country><country name="France"><region name="Île-de-France"><name sortKey="Camoin, Luc" sort="Camoin, Luc" uniqKey="Camoin L" first="Luc" last="Camoin">Luc Camoin</name></region><name sortKey="Donny Strosberg, A" sort="Donny Strosberg, A" uniqKey="Donny Strosberg A" first="A." last="Donny Strosberg">A. 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